Eng, Rick K. Huang, Rajesh K. Javitch, Wayne A. Hendrickson, Paul A. Slesinger, Matthias Quick, Different molecule species, or molecules in different conformational states or binding configurations may co-exist. Without resolving this heterogeneity, reconstructions are blurred, and are invalid as representations of any one physical structure.
Existing, widely used approaches to resolving heterogeneity, such as Relion, are based on the assumption that the structure exists in a few discrete conformations. Beginning with a paper we published jointly with the group of Abbas Ourmazd at the University He went to the Technical University of Berlin in where he finished his Ph. He hypothesized that electrons with their much smaller wavelength could be used to image objects with much higher resolution than light microscopes.
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